Christian Diener, PhD

Lifelong relationship with microbes. Now studying them for a living.

Who am I?

Hi, my name is Christian and I am a German-born scientist leading a lab at the Medical University of Graz. I work at the interface of Microbial Systems Biology, Microbial Ecology, and Computational Biology, which means I am interested in how all of the microbes, cells and molecules around and within us interact in order to form the stable ecological systems that populate virtually every surface of our planet including ourselves. In particular, I am interested how microorganisms interact with each other and their environment and what happens if those interactions are perturbed, for instance by disease or environmental changes. I strongly believe that we can leverage medical, biological, ecological, and environmental data to help us improve human health and to conserve our planet. However this will require an equitable, just, and diverse research community and society.

I love to learn new things and tinker and especially enjoy spending time with my family and dog. I also like to experiment and play around with different electronics. I enjoy sharing what I have learned and have taught many courses in academia and tech. If I am not doing any of that you will usually find me eating, cooking or baking.

If you want to know what I am currently up to follow me on bluesky, linkedin, or see my projects on github.

What am I currently working on?

My lab at the Medical University of Graz is located within the Diagnostic and Research Institute of Hygiene, Microbiology and Environmental Medicine. We also are a part of the Austrian FWF Cluster of Excellence Microbiomes Drive Planetary Health.

We study the human gut microbiome by combining ecology, systems biology and metabolism. In particular, we investigate how microbes interact with each other, their host and their environment, and how those interactions affect health and disease. Our research includes microbial nutrient use and metabolism, the effects of microbes on dietary compounds and drugs, and the design of microbiome interventions. We combine computational approaches such as metagenomics, metabolomics and modeling with wet lab experiments, including single-cell culturomics. You can learn more about our research and our work on accessible science, software and teaching on the lab website.

Before that I studied signaling in microbial cultures and metabolic alterations in cancer (you can find more about that in my publications).

Latest publication

Phenotypic heterogeneity in the human gut microbiome revealed by subspecies-resolution single-cell transcriptomics

preprint

Most of our knowledge about bacterial functional roles in microbiomes comes from bulk measurements. Yet microbial communities are complex ecosystems in which functionally distinct bacterial subpopulations with unique transcriptional states emerge across environmental niches and from interactions with other community members. Such heterogeneous transcriptional states are inherently missed by bulk measurements. To address this gap, we developed multispecies microbial split-pool ligation meta-transcriptomics (metaSPLiT), a scalable, instrument-free single-cell RNA sequencing approach for the microbiome. Using metaSPLiT, we profiled healthy human fecal microbiomes and reconstructed 21,598 single cell transcriptomes belonging to 70 unique bacterial species.

Latest post

Some personal news...

life career lab

It still feels a bit unreal to write this but here it comes. After almost 6 incredible years at the Institute for Systems Biology in Seattle I will join the faculty of the Medical University of Graz early 2024 as an Assistant Professor in Computational Microbiome Science.

My lab will be part of The Diagnostic and Research Institute of Hygiene, Microbiology and Environmental Medicine and will operate within the Austrian Cluster of Excellence “Microbiomes Drive Planetary Health”. I am excited (giddy to be honest) to join the MedUni and the amazing students, faculty and administration there.